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This tool is designed to generate primers that can be used to amplify a DNA sequenced flanked by required regions for Gateway cloning. A PCR reaction with the given primers will yield a product with selected ends and is directly useable for a recombination reaction such as a BP with pDONR221. Please enter the sequence you would like to amplify (e.g. cDNA) and select which features you would like to appear in your primers.