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BiTS

Biological Tool Suite (BiTS) Online is a collection of tools that can be used in the biological sciences. The website is currently in the early stages of development.

It is recommended that you enable javascript for this website. The tools provided are intended to be usable without javascript, but may perform suboptimally.

Tools

  • Recombination allows in silico recombination prediction of BP and LR Gateway reactions based on currently available commercial systems. Multisite recombination is currently unavailable.
  • Primer generator generates primers for cloning CRISPR guide RNAs into pX series vectors using the BbsI site as published by the Zhang lab.
  • attB Primer generator can be used to amplify a DNA sequenced flanked by required regions for Gateway cloning. A PCR reaction with the given primers will yield a product with selected ends and is directly useable for a recombination reaction such as a BP with pDONR221.
  • Complement & Translation

Tools (Beta)

  • Centrifuge Calculator allows for the selection of a centrifuge model, or manual input of centrifuge parameters. Once a centrifuge is defined this tool can then be used to calculate required RPM based on G force (RCF), G (RCF) based on RPM, as well as K factor.
  • Centrifuge Comparison extends the centrifuge calculator, providing the same functionality but with the ability to convert time on one centrifuge to time on a second centrifuge through use of K factors.

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